"Activated plasmin is a 2-chain plasmin molecule held together by 2 disulfide linkages, with 5 kringle domains. Introduction of elastase removes the N-terminal domain and four kringles leaving miniplasminogen. Removal of the last kringle leaves the lone proenzyme domain, microplasminogen. Cleaving micro-plasminogen produces micro-plasmin."
Genetically Modified: Microplasmin is a molecule created from plasminogen, one of many substances involved in blood clotting and the natural management of blood clots in the human body. PLasmin, a human serine protease, is truncated to leave microplasmin (or ocriplasmin). Microplasmin is now being bio-engineered through a genetic modification process, so that ocriplasmin (new name) can be manufactured cheaply. Currently back of the eye disorders are treated with a routine, simple surgical procedure but if clincial trials can convince regulators to permit microplasmin to be marketed as an alternative treatment, the drug companies can make a small fortune. This move would take marketshare away from certified eye surgeons, to support practitioners known as "retinal specialists".
microplasmin in fibrinolysis
from "Hydrolysis of polymeric fibrin by plasmin, miniplasmin, microplasmin and trypsin" by Andrianov SI, Makogonenko EM, Kudinov SA., 1992:
125I-labeled polymeric fibrin hydrolyzed with plasmin, Val442-plasmin (miniplasmin, Lys530-plasmin (microplasmin) and trypsin has been studied for radioactivity of its separate electrophoretic bands. The reaction of hydrolysis was stopped at a moment of a two-fold decrease of the fibrin clot turbidity (t1/2) at the wave length 350 nm. For plasmin, miniplasmin, microplasmin and trypsin taken in the same caseinolytic activities t1/2 was 12.4, 40.0 164.1 and 76.8 min, respectively. Differences in composition of fibrin digests taken at t1/2, are demonstrated: the content of high-molecular components of digests decreases in the order of plasmin greater than miniplasmin greater than microplasmin greater than trypsin, thus showing differences in the processes of fibrin clot structure disruption by the enzymes.